The conduit system of B-cell follicles is sparse and poorly branched. Peripheral LNs (except mesenteric) were collected from WT mice and cut in 40-μm-thick sections. LN sections were stained for B220 (light blue), CD3 (deep blue), ERTR-7 (red), and collagen IV (green) expression to reveal the complex structure of the conduit network present in the T- and B-cell zone when analyzed by confocal microscopy. Representative LN sections show the differences of the conduit network in the B- and the T-cell zones, as highlighted in the insets showing enlarged views of the conduits in the 2 areas. Data were acquired using a Leica Sp5 microscope (×20 and ×63 objectives) and are representative of 3 different experiments. Scale bar: 100 μm.