The conduit network is remodeled within developing B follicles. B cell–deficient (μMT) mice were injected with 2 × 10⁷ CMFDA-labeled WT B cells. Peripheral LNs (except mesenteric) were collected from WT mice (A) and μMT mice 1 day (B) and 2 weeks (C) after transfer. Sections were stained for B220 (white), CD3 (blue), ERTR-7 (green), and Lyve-1 (red) to reveal the status of the conduit network present in the T- and B-cell zone when analyzed by confocal microscopy. Histograms indicate the percentage of B-cell areas and T-cell areas occupied by the reticular fibers in each condition. Data were acquired using a Leica Sp5 microscope (×20 and ×63 objectives) and are representative of 3 different experiments. Scale bar: 100 μm.