MAL nuclear localization after Rho GTPase activation in MKs. (A) MAL cytoplasmic localization in serum-starved NIH3T3 cells and its nuclear localization after serum stimulation. (B) Cord blood-derived MKs (upper panels) and cytapheresis-derived MKs (lower panels). After adhesion to polylysine for 10 minutes (PLL, 10′), MAL is localized in the cytoplasm. After adhesion to collagen I or convulxin for 2 hours, MAL is localized in the nucleus. Both substrates induce Rho GTPase activation. MAL (red) and 4,6-diamidino-2-phenylindole (blue) staining was detected by immunofluorescence and visualized under a fluorescent light microscope at an original magnification ×60.