TC-PTP expression correlates to MYC overexpression. (A) TC-PTP and MYC overexpression in human BL cell lines. Quantitative PCR analysis of MYC () and TC-PTP () expression relative to GAPDH expression. Shown are wild-type B cells, RAJI and RAMOS BL cell lines, Val and SudHL-6 GC-like DLBCL cell lines, and OCI-Ly3 ABC-like DLBCL cell line. Error bars represent SD for 2 replicates; n = 3 or more. (B). TC-PTP expression is reduced after loss of MYC overexpression. FLL cell lines, derived from MMTV-rtTA/TRE-MYC/BCR^HEL/sHEL mice, have a doxycycline-repressible MYC promoter. Three different FLL cell lines (-21, -42, and -44) were treated with doxycycline to turn off MYC overexpression, after which mRNA was harvested and cDNA produced. Quantitative PCR analysis was performed relative to GAPDH for the following genes: MYC (top left panel), ODC (bottom left panel), TC-PTP (top right panel), and CD45 (bottom right panel) expression relative to GAPDH and normalized to untreated cells. Error bars show SD for 2 replicates; n = 4 for MYC and GAPDH, and n = 2 for ODC and CD45.