Impaired platelet surface P-selectin expression is unlikely due excessive shedding. (A) Wild-type and Fg^−/− platelets were incubated with or without either EDTA or GM6001 before addition of thrombin or TRAP. P-selectin expression on the platelet surface was analyzed via flow cytometry. The mean MFI ± SEM from the results was plotted. No significant difference in platelet surface P-selectin expression was observed on Fg^−/− platelets in the presence or absence of EDTA or GM6001. (B) Soluble P-selectin in blood plasma was detected by ELISA. There was no significant difference between Fg^−/− and wild-type mice (n = 3; P = .26).