Expression of Bcl2 family members in BMS-214662–induced apoptosis. (A) Time course of treatment with BMS-214662 (250nM) in CML CD34⁺ cells showed changes in protein levels after Western blotting analysis (n = 3). (B) Treatment with BMS-214662 or BMS-225975 (250nM) for 24 hours did not show changes in Mcl1 and Bim levels (n = 3). SDS gel 4% to 15%. (C) Activation of caspase-3 and caspase-8 in CML CD34⁺ cells measured after treatment with BMS-214662 and BMS-225975 (250nM; n = 3).