Analysis of CpG methylation for selected stemness-related genes. Mean log₂ ratios of plots for CD34⁺/CD34⁻ of 11 stemness-related genes (Table 1). (A) Log₂ ratios with respect to the distance from the TSS for transcription targets of known pluripotency-related factors, NANOG, OCT4, and SOX2 (average of 4 experiments). Positive log₂ ratios around the TSS indicate that this region is less undermethylated. (B) Mean log₂ ratio plots for PcG-regulated genes, including the genes identified as targets of Suz, Eed, and H3-lysine 27, show significant enrichment of negative log₂ ratios in downstream flanking regions of the TSS. A characteristic right shift indicates an undermethylation dip in the downstream flanking region of TSS for PcG-regulated genes.