Negative ion MALDI-TOF mass spectrometric profiles of O-glycan alditols and N-glycans released from L-selectin–bound (L-B-CD34) and –nonbinding (L-NB-CD34) forms of CD34. O-glycan alditols released from L-NB-CD34 (A) and L-B-CD34 (B). N-glycans released from L-NB-CD34 (C) and L-B-CD34 (D). Based on further structural analysis of O-glycan alditols, only the most abundant isomeric structures are shown, but other isomeric structures may also be present (see supplemental document 1 for details). Panel E summarizes O-glycan structures containing 6-sulfo SLe^x epitope identified in the present study from L-B-CD34. Molecular ions ([M-H]⁻) in panel E refer to original nondigested O-glycan alditols of panel B. Carbohydrate symbol representation shown is according to nomenclature suggested by the Consortium for Functional Glycomics (http://www.functionalglycomics.org). All molecular ions are in [M-H]⁻ form.