American Society of Hematology

Figure 4

$Figure 4. PNGase F–treated CD34 binds to L-selectin. PNGase F–digested and –nondigested CD34 was separated on L-selectin affinity chromatography, and fractions 3 (L-NB-CD34) and 13 (L-B-CD34) from both runs were analyzed on a 8% SDS-PAGE gel under reducing conditions, transferred onto the nitrocellulose filter, and immunostained using MECA-79 mAb.$

PNGase F–treated CD34 binds to L-selectin. PNGase F–digested and –nondigested CD34 was separated on L-selectin affinity chromatography, and fractions 3 (L-NB-CD34) and 13 (L-B-CD34) from both runs were analyzed on a 8% SDS-PAGE gel under reducing conditions, transferred onto the nitrocellulose filter, and immunostained using MECA-79 mAb.

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