Normal hematopoiesis in adult gata1T301K/vlt fish. (A-B) FACS analysis of adult kidney cells. (A) Representative blood cell profile of whole kidney marrow from wild-type and gata1T301K/vlt adult fish. Blood cell lineages are represented as: R, red blood cells; M, myelomonocytes; P, precursors; and L, lymphocytes. (B) Average cell numbers of each blood cell lineage calculated from 8 wild-type, 5 gata1T301K/T301K, and 5 gata1T301K/vlt fish. The differences in lineage cell numbers observed between wild-type and the mutant fish were not statistically significant. RBC indicates red blood cells. (C) Cytospin preparation from wild-type and gata1T301K/vlt adult fish whole kidney marrow, showing the presence of multilineage cells in the gata1T301K/vlt fish.
