Phenotype and expression of homing molecules of B cells and PCs generated in the 3-step culture system. MBCs were cultured as described in Figure 1. Cells were stained for CD20, CD38, and CD138. The cell phenotype was analyzed by gating on CD20⁺CD38⁻ lymphocytes, CD20⁻CD38⁺⁺CD138⁻ D7 PBs, and CD20⁻CD38⁺⁺CD138⁺ D10 PCs. (A) Black histograms represent FACS labeling with anti-CD19, CD27, CD45, HLA class II, Ki-67 (after cell permeabilization), and CD43. Gray histograms represent the corresponding negative control mAbs. Data from 1 experiment representative of 3 are shown. Numbers in panels indicate mean values ± SD of the percentage of positive cells of 3 separate experiments, and numbers in brackets indicate the mean staining indexes ± SD. (B) Black histograms represent FACS labeling with anti-CXCR5, CXCR4, CCR10, and CD62L mAbs. Gray histograms represent the corresponding negative control mAbs. Data from 1 experiment representative of 3 are shown. Numbers in panels indicate mean values ± SD of the percentage of positive cells, and numbers in brackets the mean staining indexes ± SD of 3 separate experiments. *Value is different from that in D0 MBCs using a paired t test. **Value is different from that in D4 actBCs. ***Value is different from that in D7 PBs.