cAMP inhibits the IR-induced Puma and Bax expression, mitochondrial membrane depolarization, and cleavage of caspase 9, caspase 3, and PARP. (A) Reh cells were treated as described in the legend to Figure 4A, harvested at the indicated times, and analyzed by immunoblotting with the indicated antibodies. The figure shows 1 representative experiment of 3. (Right panel) The densitometric analysis of the Puma and Bax bands at 12 hours after IR with the value obtained for untreated cells set as 1 (n = 3). (B) Reh cells were treated as described in the legend to Figure 1C. Eighteen hours after IR, cells were examined for Δψm using JC-1 (n = 4). *P < .01 relative to cells treated with IR only. (C) Reh cells were treated as described in the legend to Figure 4A, harvested at the indicated times after IR, and examined by immunoblotting with the indicated antibodies. The figure shows 1 representative experiment of 3.