NY1DD mice have increased numbers of activated pulmonary iNKT cells that are hyper-responsive to hypoxia/reoxygenation. (A) Representative flow cytometry plots of pulmonary iNKT cells. (B) iNKT cells were identified from the live, CD45⁺ lymphocyte gate as CD1d-tetramer⁺ CD3⁺ cells. Compared with C57BL/6 mice, NY1DD mouse lungs have a higher number of pulmonary iNKT cells and hypoxia/reoxygenation amplifies this response. (C-D) iNKT cells from NY1DD mice are more activated as defined by higher percentage of surface CD69 and intracellular IFN-γ that is amplified by hypoxia/reoxygenation. Data were analyzed by one-way ANOVA with Neuman-Keuls posttesting (*P < .05 was considered significant). SSC, side-scatter; H/R, hypoxia/reoxygenation.