The expression of MCL-1 in FLT3-ITD and FLT3-WT AML cells. The levels of Mcl-1 transcripts and proteins in AML bone marrow cells. (A) AML cells express ∼ 5-fold higher levels of MCL-1 compared with normal BM cells irrespective of their FAB types by real-time PCR assays. (B) FLT3-ITD AML cells expressed ∼ 2-fold higher levels of MCL-1 in average compared with those in FLT3-WT AML cells. (C) The level of MCL-1 protein on Western blot analysis in primary AML cells. Results are shown as mean ± SD (*P < .05). (D) Representative Western blot data of MCL-1 in FLT3-WT AML (cases 1, 5, and 8) and FLT3-ITD AML (cases 23, 28, and 29) samples. Vertical lines have been inserted to indicate a repositioned gel lane.