Figure 4
VEGFR-2 signaling is dependent on Ezrin binding to CD44. (A) HEK293 cells transiently transfected with a VEGFR-2 expression construct were cotransfected with vectors expressing either the CD44 cytoplasmic domain (CD44cyt) or the CD44 cytoplasmic domain mutated in the Ezrin-binding site22 (CD44mutcyt). Activation of VEGFR-2 on induction with VEGF (as described in Figure 2C) and signaling to Erk were determined. (B) HEK293 cells transiently transfected with a VEGFR-2 expression construct were cotransfected with vectors expressing EzrinΔABD as indicated, and signaling to Erk on VEGF treatment was determined. The numbers indicate fold induction. Vertical lines have been inserted to indicate repositioned gel lanes.
