Global H3K79 hypomethylation in CALM-AF10 leukemic cells. (A) H3K79 methylation in leukemia cells with CALM-AF10 rearrangement. Whole-cell extracts were prepared from AML patient samples with normal karyotype or the CALM-AF10 translocation and analyzed by Western blotting with an antibody specific for dimethylated H3K79. Detection with α-H3 antibody (bottom row) provided a loading control. (B) H3K79 methylation in the U937 cell line that expresses the CALM-AF10 fusion. The other 2 monocytic cell lines, HL60 and THP1, were used for comparison.