Type 17–polarized CD8⁺ T cells produced IL-17 and were diverted from cytolytic differentiation. Pmel-1/Thy1.1 CD8⁺ T cells were primed in IL-17–polarizing conditions or in nonpolarizing conditions and then expanded with IL-2. (A-D) The cells were then cocultured overnight with target splenocytes pulsed with hgp100_25-33 peptide. Production of IL-17, CCL20, IFN-γ, and IL-2 was determined by enzyme-linked immunosorbent assay. (E) Four-hour ⁵¹Cr release cytolysis assays were performed. Target cells were pulsed with the peptide indicated in the figure legend. Error bars indicate the SEM. (F) Real-time reverse-transcribed polymerase chain reaction was performed to assess expression of the indicated transcription factors. Expression relative to β-actin is displayed. Error bars represent the SEM.