Type 17–polarized CD8⁺ T cells mediated enhanced antitumor immunity and demonstrated greater persistence. A total of 10⁶ pmel-1/Thy1.1 CD8⁺ T cells were adoptively transferred into mice bearing established, vascularized B16F10 melanomas. Recipient mice were pretreated with 5 Gy total body irradiation, and they received adjuvant vaccine and IL-2 in conjunction with cell therapy.¹⁶  (A) Serial tumor measurements were obtained and tumor areas were calculated. Error bars indicate the SEM (N = 5). The experiment shown is representative of 3 independent experiments. (B) Expansion and persistence of adoptively transferred cells. The spleens of 3 mice per condition were examined at each time point. Error bars represent the SEM. (C-D) Expression of killer cell lectin-like receptor G1 and IL-7Rα by transferred cells after infusion as determined by flow cytometry gated on Thy1.1⁺ cells. The data represent 3 spleens per condition at each time point. Error bars represent the SEM. (E) Flow cytometric determination of intracellular cytokines after infusion. The dot plots are gated on Thy1.1⁺ cells from a pool of 3 spleens per condition at each time point.