Highly reactive transferred cells traffic to and destroy melanoma tumors in patients. (A) Sequential biopsies of subcutaneous tumors from DMF5 patient 4 before (d0) and after treatment, stained with hematoxylin and eosin (left), or anti-CD8 (right). Original magnification ×20. (B) DMF5 patient 4 thigh covered with multiple subcutaneous melanoma lesions before treatment, and after partial tumor regression 5 weeks after treatment. (C) Flow cytometry of TILs grown from DMF5 patient 4 subcutaneous tumor resected 4 weeks after treatment. (D-F) Before treatment and after treatment CT scans of (D) DMF5 patient 2 lungs, (E) DMF5 patient 8 brain (top) and axilla (bottom), and (F) gp100(154) patient 14 lungs (top) and liver (bottom). Arrows represent location of melanoma metastases.