Increased erythroid differentiation of hematopoietic progenitor cells generated from PV iPS cells. To assess erythroid differentiation potential, purified CD34⁺CD45⁺ cells from both normal control (NC) and PV iPS cells after EB-mediated hematopoietic differentiation were plated into a liquid culture medium (A,C), or a serum- and methylcellulose-containing medium (B,D). (A) Fold of cell expansion after 7 days of the liquid culture from the purified CD34⁺CD45⁺ cells derived from NC (13.5 ± 3.6-fold) or PV iPS cells (27.6 ± 3.3-fold). (B) Fold of cell expansion of the CD34⁺CD45⁺ cells after 14 days of the methylcellulose culture, 69.5 ± 24.7-fold of NC versus 127 ± 28.3-fold of the PV iPS cells. Data in panels A and B are presented as mean ± SD (n = 2). (C) FACS analysis of the 7-day cultured cells for the erythroid phenotype (CD235a⁺CD45⁻). The percentages of such cell population are indicated in the top left quadrant based on the gating and comparison with background staining. (D) FACS analysis of the 14-day cells harvested from the methylcellulose-containing medium.