Human iPS generated hematopoietic progenitor cells exhibit unique gene expression pattern similar to the primary CD34⁺ cells from the PV patient and a normal control. (A) Total RNA was isolated from primary CD34⁺ cells from a healthy donor as an NC or from the PV patient (MPD183) where the PV-iPS cell lines were derived from. Gene expression of NFI-B, HBG, and HBB as well as β-actin (as a control) was analyzed by real-time quantitative PCR after reverse transcription of RNA. The normalized level (relative to that of β-actin) is plotted. (B) An identical analysis of purified CD34⁺CD45⁺ cells generated from PV iPS (iMPD183) cells and NC iPS cells derived from normal adult CD34⁺ cells. Data are presented as mean ± SD (n = 2).