American Society of Hematology

Figure 2

$Figure 2. Integrin expression in PBMNCs, HUVECs, and EPCs. (A) Confirmation by immunoblotting. Note that the platelet integrin GpIIb/IIIa was specifically enriched in the MP fraction of EPC cultures. Besides integrins, MPs also contained CD31 (PECAM), although at lower abundance than GpIIb/IIIa, consistent with the spectral counts presented in Table 1. Talin-1 and VASP, 2 downstream mediators of integrin signaling, showed a molecular weight shift indicative of phosphorylation. Actin was used as loading control. (B) No mRNA expression was detectable for GpIIb/IIIa in EPCs. A similar result was obtained for the platelet integrin α6. The less abundant integrins (according to the spectral counts in Table 1) were expressed at the mRNA level. The data are representative of 3 independent experiments.$

Integrin expression in PBMNCs, HUVECs, and EPCs. (A) Confirmation by immunoblotting. Note that the platelet integrin GpIIb/IIIa was specifically enriched in the MP fraction of EPC cultures. Besides integrins, MPs also contained CD31 (PECAM), although at lower abundance than GpIIb/IIIa, consistent with the spectral counts presented in Table 1. Talin-1 and VASP, 2 downstream mediators of integrin signaling, showed a molecular weight shift indicative of phosphorylation. Actin was used as loading control. (B) No mRNA expression was detectable for GpIIb/IIIa in EPCs. A similar result was obtained for the platelet integrin α6. The less abundant integrins (according to the spectral counts in Table 1) were expressed at the mRNA level. The data are representative of 3 independent experiments.

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