Figure 1
Analysis of LCL-reactive T cells in peripheral blood using the ELISpot assay of IFNγ release. Ex vivo PBMCs from EBV-seropositive donors (P1-P5) or EBV-seronegative donors (N1-N5) were CD56-depleted and then either (A) CD8-enriched by CD4+ T-cell depletion or (B) CD4-enriched by CD8+ T-cell depletion. These preparations were seeded at 1 × 105 and 2 × 105 cells/well in duplicate wells and cocultured with autologous B95.8 and BZ k/o LCLs (grown for at least 1 month in HuS) at an effector-to-target ratio of 40:1. Results from one representative experiment are expressed as mean ± 1 SD spot-forming cells (SFCs) per 2 × 105 cells; background responses from nonstimulated cells are also shown (control). *P < .05 (Wilcoxon-Mann-Whitney test).
