Expression of CD20 on normal B cells alters the trafficking and survival of CD20-specific cTCR⁺ T cells. (A) Bioluminescent imaging of 10⁷ CBR⁺Leu16⁺Thy1.1⁺CD8⁺ T cells 4 hours after intravenous injection into WT or hCD20 mice. (B) The lung-to-nonlung bioluminescent ratio was calculated for mice injected with 10⁷ CBR⁺Leu16⁺Thy1.1⁺CD8⁺ T cells after various time intervals. Data from 2 experiments were overlaid, comprising 7 to 10 mice per time point. Red outlines in panels A and C are representative of regions of interest for signal quantitation. (C) Bioluminescent image of mice 48 hours after T-cell injection. (E) Serial quantitation of T-cell bioluminescent signal from the femurs in panel D, and the entire mouse in panel E was performed over time. (F) The relative T-cell signal originating from the femurs was calculated by division of total signals from femurs by total signals from mice. The data are representative of 2 experiments with 3 to 5 mice per group each. Data points represent mean ± SE. P values were obtained by 2-tailed t tests. Bioluminescent images were assembled with the use of Illustrator (Adobe Systems).