Anti–LFA-1 enhances accumulation of CD20-specific T cells in the BM but not LNs. (A-B) A total of 10⁷ CBR⁺Thy1.1⁺cTCR⁺CD8⁺ T cells pretreated with either anti–LFA-1 or PBS were injected intravenously into WT mice. Serial bioluminescent imaging of T cells was performed, and the relative signals emitted were calculated by dividing the signal from the femurs in panel A or cervical LN (cLN) in panel B by the total bioluminescent signal. Data points represent the mean ± SE. Figures contain data from 2 experiments with 7 to 8 mice per group total. P values were obtained by 1-way ANOVAs, followed by a Bonferroni selected comparison test. (C) Depiction of cervical LN and femur gates from representative mice.