B-cell depletion with anti-hCD20 antibodies promotes CD20-specific cTCR⁺ T-cell survival and function. (A) Calculation of lung-to-nonlung bioluminescent signal ratio from mice injected intravenously with 2 × 10⁷ CBR⁺cTCR⁺Thy1.1⁺CD8⁺ T cells. Data from 2 experiments were overlaid, comprising 7 to 8 mice per time point. (B) Serial quantitation of T-cell bioluminescent signal from mice injected with cTCR⁺ T cells. Data are representative of 2 experiments with 3 to 4 mice per group. Data points represent mean ± SE. (C) Bioluminescent image of mice from panel B 72 hours after injection. (D-F) Five days after CBR⁺cTCR⁺Thy1.1⁺CD8⁺ T cells were injected into untreated or B cell–depleted mice, spleens were harvested, and the remaining CD19⁺ B cells were enumerated. (D) 1F5 (200 μg) was injected into hCD20 mice 4 days before T-cell injection. Horizontal lines represent the mean of the data. (E) Quantitation of MZ and follicular (FO) B cells. Bar graphs represent mean ± SE. Combined data from 2 experiments with 3 to 4 mice per group are shown (D-E). Bar graphs and data points represent mean ± SE. (F) Magnification of panel E. P values were obtained by 1-way ANOVA with a Tukey post hoc test. Bioluminescent images were assembled with the use of Illustrator (Adobe Systems).