Real-time PCR validation of miR-146a and miR-155 expression. Relative FC of expression in stimulated samples against untreated controls were calculated using the comparative Ct (2^−ΔΔCt) method. Let-7a was chosen as the endogenous control because it displayed more constant expression values among all treatments with respect to U6 (data not shown). PCRs were run in triplicate, and the mean of 4 independent experiments ± SD is shown. P values were calculated by ANOVA, and statistical significance is indicated comparing results from MD-DCs treated with a combination of agonists versus the single treatments.