Enhanced development of γδ T cells in the absence of Itk is bone marrow intrinsic. Thy1.1⁺WT or Thy1.1⁺Itk^−/− bone marrow was intravenously injected to irradiated Thy1.2 WT and Itk^−/− mice. Eight weeks later, the percentage of CD3⁺γδTCR⁺ cells of donor WT and Itk^−/− cells (A) and the percentage of CD4 and CD8 population on donor γδ T cells (B) were analyzed. Data are representative of 2 independent experiments. (C) The 1:1 mixtures of bone marrow from CD45.1/Thy1.2 congenic WT mice and CD45.2/Thy1.2 congenic Itk^−/− mice were intravenously injected to irradiated Thy1.1 WT mice. Eight weeks later, the percentage of CD3⁺γδTCR⁺ cells of donor WT and Itk^−/− was analyzed. Data are representative of 3 mice.