E-cadherin cell surface expression is modulated by its cytoplasmic domain. (A) Schematic representative of E-cadherin constructs. (B) Parental or the various E-cadherin transfectants as indicated were stained with KLRG1 tetramer or E-cadherin antibody and analyzed by FACS. Data are representative of at least 5 independent experiments. (C) Cell lysates were blotted for Myc and reprobed with β-actin. Data are representative of at least 4 independent experiments. Quantitation was performed as described in “Western blotting and quantitation.”