E-cadherin is tyrosine phosphorylated when bound to KLRG1.(A) Parental or WT E-cadherin–transduced cells were treated with pervanadate or left untreated and E-cadherin was immunoprecipitated from lysates. Immunoprecipitated proteins were analyzed by Western blot using the indicated mAbs. (B) E-cadherin transfectants were incubated at different time points with parental or KLRG1 chimeric receptor reporter cells. E-cadherin immunoprecipitated lysates were resolved by SDS-PAGE and transferred to nitrocellulose membrane for Western blot analysis with anti-pY mAb 4G10. The results are representative of at least 5 independent experiments.