Anti-EMMPRIN antibody inhibits VEGF isoforms and VEGFR-2 expression induced by EMMPRIN. (A) HMEC-1 cells were incubated with anti-EMMPRIN blocking antibody or with an IgG control antibody in serum-free medium before the addition of Emp for 48 hours, and VEGF in the CM was quantified by ELISA. Results were normalized to the cell number and expressed as pg/10⁶ cells. Columns indicate means of 3 independent experiments; and bars, SD. *P < .05. (B) HMEC-1 cells were incubated for 2 hours with 20 μg/mL anti-EMMPRIN blocking antibody or with an IgG control antibody in serum-free medium. VEGF₁₂₁, VEGF₁₆₅,VEGF₁₈₉, and VEGFR-2 transcripts were quantified by qRT-PCR. Columns indicate means of relative expression to TBP housekeeping gene of at least 3 independent experiments; and bars, SD. *P < .05.