Role of HIFs-1α and -2α in the hypoxic induction of other key genes by macrophages. (A,C,E,G,I,K) Hypoxic induction of mRNA for CXCR4, GLUT-1, ADM, STAT4, ADORA2A, and ICAM1 (as measured by quantitative RT-PCR) after exposure of primary human macrophages to normoxia (20.9% O2; N) or hypoxia (0.1% O2; H) for 18 hours, or hypoxia after exposure to siRNA for HIF-1 α (1 α), HIF-2 α (2 α), both HIFs-1 α and -2 α together (1 α + 2 α), or a scrambled control (Scr). (B,D,F,H,J,L) Hypoxic induction of the same genes in wild-type or HIF-1α–deficient murine BMDMs. Pooled data from 3 replicate experiments are shown. *P < .05 compared with corresponding normoxic group. ∧P < .05 compared with the Scr siRNA/hypoxia group. +P < .05 compared with macrophages from wild-type mice exposed to hypoxia.
