Skin-derived DCs in the draining lymph nodes are significantly reduced in GM-CSF– and FL-deficient mice in steady state. (A) Flow cytometry and analysis of DC subsets in skin-draining LNs (pooled inguinal, axillary, and cervical LNs). Horizontal lines indicate mean values of the results. LN DC subsets gated as CD40^hiCD11c^int, CD40⁺CD11c^hi, and CD40⁻CD11c^int. Total LN cell numbers (A) and percentage of each LN DC subset (B) are shown in graphs. *P < .05; **P < .01.