MiR29a and -29b targets are specifically and significantly down-regulated after miR-29s transfections. (A) Venn diagram showing common probes between miR-29a and -29b Affymetrix signatures. Microarray mRNA profiling (Affymetrix) was performed using RNA obtained from K562 cells transfected with either synthetic miR-29a (n = 2) or miR-29b (n = 4) and scrambled oligonucleotides (n = 4). Class comparison analysis (miR-29b vs scrambled and miR-29a vs scrambled) were performed using P less than .001. The number in the intersection of the Venn diagram represents the number of probes that are shared between miR-29a and -b signatures, whereas the numbers in the left and right circle are the probes that are only differentially expressed in miR-29a and -b, respectively. (B) The box plots display the median and SD of t test score distributions in the 2 groups of Affymetrix experiments: targets of miR-29 or all other nontargets genes. The open circles represent the genes with t test score higher than 95th percentile in each individual distribution. The t test scores are positive for genes up-regulated by miR-29 transfections, whereas negative scores belong to down-regulated genes.