The pdFVIII and rFVIII treatments induce the expression of different genes in DCs. Gene expression in DCs 24 hours after the (A) rFVIII or (B) pdFVIII treatments was analyzed via quantitative RT-PCR. The HBSS-treated mice were used as a control. The fold change for each gene was calculated as explained in “Quantitative real-time PCR.” Fold inductions were considered statistically significant if the P value was less than .05 in at least 3 of the 4 biologic repeats. The dashed line represents the gene expression of the control samples. The error bars represent the SEM. N = 4