p210 leukemia is sensitive to GVL mediated by donor-derived TNF and LTα3. (A) Leukemic relapse by Kaplan-Meier analysis in lethally irradiated (1100 cGy) B6C3F1 recipients transplanted with 5 × 10⁶ BM and 0.5 × 10⁶ T cells from B6.WT, B6.Lta^−/−, B6.Tnf^−/− or B6.Tnf/Lta^−/− mice or 5 × 10⁶ B6.WT TCD BM, in conjunction with 1 × 10⁶ p210 tumor cells (H-2D^k, luciferase⁺). P = .024 Lta^−/− vs Tnf/Lta^−/−. P = .048, Tnf^−/− vs Tnf/Lta^−/−. P < .0001 Tnf/Lta^−/− vs WT. P = .048 Tnf^−/− vs WT, T cell–replete groups. Data are combined from 2 replicate experiments, n = 16 in BM + T groups and n = 10 in TCD control. (B) Representative bioluminescent imaging, taken at day 19 after transplantation.