IFNβ induces caspase-11 expression and active caspase-3 in mature DCs. (A) B10.A DCs were treated with IFNβ or matured with the cytokine cocktail in the presence or absence of IFNβ (1000 IU/mL) and analyzed for caspase-11 expression by real-time RT-PCR (24 hours; top panel) and for levels of active caspase-3 by flow cytometry (48 hours; bottom panel). (B) wt and STAT-1^−/− DCs were treated as in panel A and analyzed for caspase-11 expression and active caspase-3 levels. (C) wt and STAT-1^−/− mice were matured with the cytokine cocktail in the presence or absence of IFNγ (100 ng/mL) and analyzed for levels of active caspase-3 by flow cytometry. Data are representative of 3 independent experiments.