IFNβ-induced DC apoptosis requires caspase-11 expression. CD11c⁺ DCs generated from wt or caspase-11^−/− mice were treated with IFNβ (1000 IU/mL) or the cytokine cocktail in the presence or absence of IFNβ (1000 IU/mL). Caspase-11 mRNA expression was determined by real-time RT-PCR (24 hours); active caspase-3 levels and apoptosis were determined by FACS (48 hours). Data are representative of 2 independent experiments with triplicate samples.