Asxl1^tm1Bc/tm1Bc mutant mice exhibit splenomegaly and increase in myeloid cell number. (A) Cellularity of Asxl1^tm1Bc/tm1Bc (mut) adult spleens is increased compared with wild type (wt; n = 14 wt, 12 mutant). (B) In adult Asxl1^tm1Bc/tm1Bc mice, the absolute numbers of myeloid (Gr1 or Mac1 positive) and erythroid (Ter119 positive) cells are increased in spleen (Gr1⁺ n = 13 wt, 11 mutant; Mac1⁺ n = 11; Ter119⁺ n = 10 wt, 7 mutant). (C) The proportion of cells bearing the myeloid lineage markers Gr1 (i) and Mac (ii) is increased in adult spleen of Asxl1^tm1Bc/tm1Bc mutants.