Mobilization of hematopoietic progenitors in splenectomized mice. (A) Wild-type and splenectomized C57BL/6J x 129Sv/J F1 mice were treated with G-CSF 250 μg/kg per day subcutaneously × 5 days, plerixafor 5 mg/kg subcutaneously, BIO5192 1 mg/kg intravenously, or the combination of plerixafor 5 mg/kg and BIO5192 1 mg/kg. Treatment in splenectomized mice began 7 days after splenectomy. Peripheral blood was assayed for CFU-GM on day 5 for G-CSF–treated mice, 1 hour after injection for BIO5192, and 3 hours after injection for plerixafor and plerixafor + BIO5192–treated mice (n = 6-9 each group). Data are mean ± SEM of (A) absolute numbers of CFU/mL peripheral blood or as (B) fold increase relative to CFU/mL peripheral blood immediately before treatment. *P < .05; **P < .01; ***P < .001.