Stable transgene expression in NK cells differentiated from SB100X-transfected CD34+ HPCs. Transfected CD34+ cells were cocultured with irradiated AFT024 cells and the indicated cytokines. On day 28, cells were harvested and analyzed by flow cytometry. DsRed+ cells were derived from SB transposon + SB100X-transfected CD34+ HPCs after coculture on AFT024 stromal cells.