Ox-DCs have a limited ability to mature. Immature DCs (thin black line) or DCs differentiated in the presence of 40 μg/mL OxPAPC (gray histogram) were stimulated with LPS (100 ng/mL; A) or PGN (1 μg/mL; B, thick black line) for 24 hours, harvested, and stained for the indicated markers and measured by flow cytometry. Purified T cells were stimulated with graded numbers of allogenic immature DCs or DCs differentiated in the presence of 40 μg/mL OxPAPC (left) or the indicated amounts of OxPAPC (right) and stimulated with LPS (C) or PGN (D), and the proliferation of T cells was monitored on day 5 of culture by adding [methyl-³H]thymidine followed by measuring [methyl-³H]thymidine incorporation 18 hours later.