Ox-DCs produce less IL-12. (A-F) Immature DCs or DCs differentiated in the presence of 10, 25, or 40 μg/mL OxPAPC were stimulated with LPS or PGN. At 24 hours later the supernatants were harvested and analyzed for IL-12p40, IL-12p70, and TNF by ELISA. Data are representative of 3 independent experiments. (G-H) Supernatant of MLR where DCs and Ox-DCs stimulated with LPS or Poly I:C were used as stimulators was taken and analyzed for IFN-γ by ELISA. Data are representative of 2 independent experiments.