Cytokine secretion profiles for early lots of IL-17–secreting clones. Resting T-cell clones (first seed lot for 17A-19WK-11 and second seed lots for 17A-19WK-22 and -33) were stimulated with FVIII_2194-2213 (0.1, 1.0, and 10 μM) presented on irradiated PBMCs from an unrelated DRB1*0101 donor. Cell supernatants were collected from triplicate wells after 48 hours to measure IFN-γ, IL-4, and IL-17 by ELISA. The cytokine levels of cells stimulated with buffer as a negative control were subtracted.