Rag1-GFP^low cells retain a minimal residual myeloid potential. (A) Percentage of seeded single cells reaching a given clone size as estimated by visual scoring using light microscopy (“In vitro evaluation of myeloid potential”). Cells were sorted based on the expression of surface markers and reporter genes. LMPPs were sorted as LIN^−/lowSCA1^highKIT^highFLT3^high, whereas CLP (Rag1^low, Rag1^high, and λ5Tg⁺) and FrA (Rag1^high and λ5Tg⁺) subpopulations were sorted as shown in Figure 1A, Each diagram is associated with results from gene expression analysis (B). A set of randomly chosen clones from the different experiments were investigated by multiplex RT-PCR. The dots indicate detected expression of the indicated gene. Each column represents one cell. Data in graphs are collected from 2 independent sorting experiments and represent average (range).