Alterations in B-lineage gene expression patterns in CLP subpopulations are associated with reporter gene expression. (A) Result of a gene expression microarray analysis comparing the gene expression patterns in Rag1^low and Rag1^high LIN⁻FLT3⁺IL-7R⁺SCA1^lowKIT^low CLPs. Cells used for replica arrays originated from independent sorts, and cluster analyses have been done using genes with at least 2.5-fold difference in expressed between either of the cell populations. Red represents high expression; and blue, low expression. Calculated RMA expression values are indicated. (B) Quantitative PCR data where the relative expression of a set of genes is analyzed in purified cells. LMPPs were sorted as LIN^−/lowSCA1^highKIT^highFLT3^high, whereas CLP (Rag1^low, Rag1^high, and λ5Tg⁺) and FrA (Rag1^high and λ5Tg⁺) subpopulations were sorted as shown in Figure 1A. The data are presented as relative expression values normalized to Hprt1 expression levels. Data are average (SD) from cells originating from 3 to 5 independent sorts.