The majority of the Rag1^high cells express the surface marker LY6D. (A) Representative FACS plots showing Rag1-GFP and LY6D expression in LIN⁻FLT3⁺IL-7R⁺SCA1^lowKIT^low CLP/FrA cells. Numbers indicate percentage of gated cells. LIN include: GR1, CD11B, CD3ϵ, TER119, NK1.1, CD19, and CD11C. (B) Rag1 quantitative RT-PCR data generated from sorted LY6D⁺ and LY6D⁻ CLPs. The data are presented as relative expression values normalized to Hprt1 expression levels, and data are collected from 3 quantitative PCR reactions. (C) Distribution of B-, B/NK-, and NK-cell containing clones generated from single LIN⁻FLT3⁺IL-7R⁺SCA1^lowKIT^low LY6D^+/− CLPs as indicated. The imbedded small diagram indicates total cloning frequency. The data are collected from 2 independent experiments, and the error bars represent variation between experiments. For sorting of LY6D^+/− CLPs, LIN include: GR1, CD11B, CD3ϵ, TER119, NK1.1, CD19, CD11C, and B220.