p110δ regulates cytokine production late after TCR stimulation. CD8⁺ OT1 (left column) or CD4⁺ OT2 (right column) T cells were stimulated with irradiated APCs plus 1mM OVA peptide or left unstimulated. A total of 10mM LY or 5mM IC was added at the start of coculture or 7 or 24 hours later. Vehicle control (DMSO) was added at the experiment start for uninhibited controls. After 48 hours, proliferation (A-B), IL-2 (C-D), and IFN-γ production (E-F) were measured. Data are mean ± SEM of triplicate readings and are representative of 2 independent experiments.