Improved T-independent and T-dependent immune responses in EμB29-Btk-LV–treated mice. Mice were immunized with TNP-Ficoll or NP-CGG in alum at 15 to 17 weeks after transplantation. (A) Sera levels of IgM, IgG₃, and TNP-IgM at day 14 after immunization were analyzed by ELISA and expressed relative to IgM or IgG₃ standard curves. (B) Sera levels of NP-IgM were evaluated by ELISA at days 0 and 7, and levels of NP-IgG were evaluated at days 0 and 14. WT and KO datasets include both unmanipulated and mock-transplanted animals. Data represent 4 independent experiments (A) and 1 experiment (B). P values compare EμB29-Btk and KO groups: ***P < .001; **P = .001-.01; *P = .01-.05.