Model of CML-like disease in mice. (A) Schema of retroviral bone marrow transduction and transplantation to irradiated recipient mice. (B) Donor bone marrow (CD45.2⁺) was transferred into CD45.1⁺ recipient mice (irradiated with 4.5 Gy). Twenty days later, PBMCs were stained for GR-1, CD8, CD4, B220, and NK1.1, and analyzed by flow cytometry. Representative dot plots of 1 of 6 mice are shown. (C) Survival of cpCML (—, n = 12) and bcCML (, n = 18) mice is shown in Kaplan-Meier plots. Statistical comparison was performed with the log-rank test. (D) Phenotypical characterization of GFP⁺ leukemic cells in cpCML and bcCML mice. Cells were gated on granulocytes in control C57BL/6 mice and on transduced granulocyte populations in cpCML and bcCML mice. One representative staining of 5 is shown.